Cell Culture Manufacturing Process

Our autologous cell cultures are produced to the highest standards in the highest possible cleanroom class A. The cultivation and provision of keratinocytes are subject to strict regulatory requirements, which require the DIZG to maintain a separate cleanroom area just for them.

From collection to dispatch

  • 1
    1. COLLECTION/PREPERATION
    • The DIZG collection kit is handed over to the clinic with biopsy tubes, documents and guidelines for collection.
    • After collection, the biopsies are sent back to the DIZG.
    • The biopsies are gently decontaminated before cell isolation.
  • 2
    2. PROCESSING
    • The cells from the biopsies are isolated and further processed within cleanroom class A, incubated in specially designed incubators at 37 °C and 5% CO₂.
    • The growth and morphology of the cells are monitored microscopically.
  • 3
    3. PREPARATION OF THE SHEETS FOR TRANSPLANTATION
    • The sheets are cultivated until a fine skin develops. This takes around three weeks on average.
    • During preparation, each sheet is detached and bound to a carrier.
    • The finished transplants are sent refrigerated by a special courier.
  • 4
    4. PREPARATION OF THE SUSPENSION FOR TRANSPLANTATION
    • The keratinocyte suspension can be prepared for transplantation after ten (+/- two) days.
    • The finished suspension is filled into syringes with a special nozzle-cap closure and dispatched.

Good results require good co-operation between the clinic and the DIZG.

1.1. Collection kit
1.1. Collection kit
1.2. Preparation of the biopsies
1.2. Preparation of the biopsies
2.1. Processing the cells
2.1. Processing the cells
2.2. Control of culture growth
2.2. Control of culture growth
3.1. Befestigung des Sheets an der Trägergaze
3.1. Befestigung des Sheets an der Trägergaze
3.2. Dispatch of the sheets
3.2. Dispatch of the sheets
4. Packaging/shipping of the suspension
4. Packaging/shipping of the suspension
autologous cell cultures

To our autologous cell culture service